what is 100um micron counting chamber slide used for？

A sperm counting chamber slide with a depth of 100 micrometers (100μm) is a specialized laboratory instrument designed for precise cell counting and analysis under a microscope. The depth of the chamber is a crucial factor, as it allows for accurate counting of cells or particles within a specific volume.

Here are some key points regarding a counting chamber slide with a depth of 100 micrometers:

1. **Depth Measurement:** The depth of the counting chamber is typically specified in micrometers (μm) and represents the height or thickness of the chamber where the sample is placed.

2. **Application in Cell Counting:** A counting chamber slide with a 100μm depth is often used for counting cells, such as in hemocytometry. The chamber's depth allows for a known volume of the sample to be analyzed, facilitating accurate cell concentration calculations.

3. **Microscopic Observation:** The counting chamber is placed on a microscope stage for microscopic observation. Grid lines or markings on the chamber aid in accurate counting.

4. **Cell Viability and Density:** Such counting chambers are commonly used to assess cell viability, concentration, and density in various biological samples, including blood, cell cultures, and other suspensions.

5. **One or Two Chambers:** Counting chamber slides may have one or two chambers, each with a specified depth. Two-chambered slides are convenient for parallel sample analysis.

6. **Cover Slip:** A cover slip is typically placed on top of the sample to create a uniform depth and to prevent sample evaporation during observation.

7. **Calibration:** Before counting cells, the counting chamber must be calibrated to ensure accurate volume measurements and counting.

It's important to note that the choice of a counting chamber depth depends on the size and characteristics of the cells or particles being counted. Researchers and laboratory professionals should select the appropriate counting chamber based on their specific needs and the characteristics of the samples they are analyzing.

Also our ASCEN Disposable 2-chamber glass slides that are a fixed depth of 100um. This slide is designed and regularly used for Post-Vasectomy analysis all around Great Britain and Europe. They are also compatible with many automated semen analysers. ASCEN Disposable Glass Chambers remove any risk of infection and save time during semen analysis.