what is the process of hospital human clinical fertility ivf laboratory casa software system sperm analysis

The process of sperm analysis using a hospital clinical fertility IVF laboratory CASA (Computer-Assisted Sperm Analysis) software system involves several detailed steps to evaluate sperm quality and fertility potential. Below is the process:

1. **Sample Collection**
   - **Collection Method**: Patients provide semen samples via masturbation in a sterile container. Abstinence of 2-5 days is usually recommended before collection.
   - **Sample Handling**: Samples must be kept at body temperature and analyzed within an hour of collection to ensure accuracy.

2. **Sample Preparation**
   - **Mixing**: The semen sample is gently mixed to ensure homogeneity.
   - **Liquefaction**: Samples are allowed to liquefy at room or incubator temperature (37°C), which typically takes 15-30 minutes.
   - **Dilution (if necessary)**: If sperm concentration is high, the sample may be diluted with a suitable medium.

3. **Loading the Sample**
   - **Chamber Slide Preparation**: A disposable sperm counting chamber slide or specialized glass slide is used.
   - **Slide Loading**: A drop of the semen sample is placed into the chamber slide, avoiding bubbles.

4. **CASA System Analysis**
   - **Placement in Analyzer**: The slide is inserted into the CASA system.
   - **Automated Analysis**: The CASA software uses advanced imaging technology to analyze multiple parameters, including:
     - **Concentration**: Total sperm count per milliliter.
     - **Motility**: Categorization of sperm into progressively motile, non-progressively motile, and immotile groups.
     - **Velocity and Kinematics**: Parameters like curvilinear velocity (VCL), straight-line velocity (VSL), and linearity (LIN).
     - **Morphology**: Detailed assessment of sperm structure, including head, midpiece, and tail abnormalities.
     - **Viability**: Optional assessment of live vs. dead sperm using specific stains.
     - **Fragmentation**: DNA integrity testing, if the CASA system includes this feature.

5. **Data Recording and Reporting**
   - **Visualization**: Real-time motility and movement patterns are displayed.
   - **Automatic Calculation**: Results are compared against WHO standards for sperm quality.
   - **Report Generation**: A detailed report is created, highlighting key metrics like total motility, morphology percentages, and recommendations for fertility treatment.

 6. **Clinical Interpretation**
   - The clinician reviews the CASA-generated report alongside the patient’s medical history.
   - Recommendations for treatment, such as IUI, IVF, or ICSI, are made based on the findings.

Advantages of CASA Systems in Clinical Settings
- **Accuracy and Standardization**: Reduces operator error by automating measurements.
- **Efficiency**: Faster analysis compared to manual methods.
- **Comprehensive Metrics**: Provides detailed and reproducible data on sperm health.
- **Integration with Fertility Labs**: Directly interfaces with other IVF laboratory equipment and data management systems.

Advanced CASA Systems in Use
Popular systems include:
- **Hamilton Thorne IVOS II**
- **Microptic SCA**
- **Origio Sperm Class Analyzer**

These systems are essential tools in male fertility assessment and IVF laboratories, ensuring precise evaluation and aiding in personalized fertility treatments.

Our ASCEN CASA disposable counting chamber slide is perfectly match with the world popular CASA Software system for automatic semen analysis.


1. Hamilton Thorne Computer Assisted Sperm Analysis
CASA (Computer Assisted Sperm Analysis) image analysis systems are designed to bring quality, efficiency, and reliability to studies of reproductive cells in the human fertility*, animal sciences, and reproductive toxicology fields. Our sperm analyzers assist researchers and clinicians in analyzing sperm motility, morphology, or other characteristics in human fertility, toxicology, research, and animal applications. IVOS II CASA Sperm Analyzer, CEROS II Microscope Sperm Analyzer, TOX IVOS II CASA Semen Analyzer

2. MICROPTIC CASA Systems SCA CASA System for semen analysis allows the accurate, repetitive and automatic assessment of the following sperm parameters: motility, concentration, morphology, DNA fragmentation, vitality, acrosome reaction and leukocytes.

3. Minitube Androscope AndroVision: CASA software with PC and monitor
AndroVision CASA Analyzer is a highly precise automated system for computerized semen analysis. It not only provides classical analyses of motility, concentration and morphology, but also various fluorescence based assessments of sperm functionality. The basic system for the analysis of motility and concentration is complemented by optional software modules.

4. computer assisted semen analysis System - IMV TECHNOLOGY
All these CASA Comptuter Assisted Semen Analysis Software System Analyzer popular in the market